Simultaneous Quantification of Serum Nonesterified and Esterified Fatty Acids as Potential Biomarkers to Differentiate Benign Lung Diseases from Lung Cancer.

ABSTRACT: In this study, we have employed graphene oxide as a matrix to simultaneously and directly quantify serum nonesterified and esterified fatty acids (FAs) using matrix-assisted laser/desorption ionization-Fourier transform ion cyclotron resonance mass spectrometry (MALDI-FTICR MS). Twelve serum nonesterified FAs combined with their individual esterified FAs (i.e., C_16:0, C_16:1, C_18:0, C_18:1, C_18:2, C_18:3, C_20:2, C_20:3, C_20:4, C_20:5, C_22:5, and C_22:6) were quantified based on their calibration curves with the correlation coefficients of >0.99, along with the analytical time of <1 min each sample. As a result, serum levels of twelve total FAs (TFAs) in 1440 serum s

SUBMITTER: Ren J 

PROVIDER: S-EPMC5043242 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature