Single-cell RNA sequencing reveals dynamic changes in A-to-I RNA editome during early human embryogenesis.

ABSTRACT:

Background

A-to-I RNA-editing mediated by ADAR (adenosine deaminase acting on RNA) enzymes that converts adenosine to inosine in RNA sequence can generate mutations and alter gene regulation in metazoans. Previous studies have shown that A-to-I RNA-editing plays vital roles in mouse embryogenesis. However, the RNA-editing activities in early human embryonic development have not been investigated.

Results

Here, we characterized genome-wide A-to-I RNA-editing activities during human early embryogenesis by profiling 68 single cells from 29 human embryos spanning from oocyte to morula stages. We demonstrate dynamic changes in genome-wide RNA-editing during early human embryogenesis in a stage-specific fashion. In parallel with ADAR expression level changes, the genome-wide A-to-

SUBMITTER: Qiu S 

PROVIDER: S-EPMC5043600 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature