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Transcriptomic and Proteomic Analysis of Oenococcus oeni Adaptation to Wine Stress Conditions.


ABSTRACT: Oenococcus oeni, the main lactic acid bacteria responsible for malolactic fermentation in wine, has to adapt to stressful conditions, such as low pH and high ethanol content. In this study, the changes in the transcriptome and the proteome of O. oeni PSU-1 during the adaptation period before MLF start have been studied. DNA microarrays were used for the transcriptomic analysis and two complementary proteomic techniques, 2-D DIGE and iTRAQ labeling were used to analyze the proteomic response. One of the most influenced functions in PSU-1 due to inoculation into wine-like medium (WLM) was translation, showing the over-expression of certain ribosomal genes and the corresponding proteins. Amino acid metabolism and transport was also altered and several peptidases were up regulate

SUBMITTER: Margalef-Catala M 

PROVIDER: S-EPMC5044463 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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