Global Transcriptional Analysis of Virus-Host Interactions between Phage ϕ29 and Bacillus subtilis.

ABSTRACT:

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The study of phage-host relationships is essential to understanding the dynamic of microbial systems. Here, we analyze genome-wide interactions of Bacillus subtilis and its lytic phage ϕ29 during the early stage of infection. Simultaneous high-resolution analysis of virus and host transcriptomes by deep RNA sequencing allowed us to identify differentially expressed bacterial genes. Phage ϕ29 induces significant transcriptional changes in about 0.9% (38/4,242) and 1.8% (76/4,242) of the host protein-coding genes after 8 and 16 min of infection, respectively. Gene ontology enrichment analysis clustered upregulated genes into several functional categories, such as nucleic acid metabolism (including DNA replication) and protein metabolism (including translation). Surprisingl

SUBMITTER: Mojardin L 

PROVIDER: S-EPMC5044823 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature