Epitope-Independent Purification of Native-Like Envelope Trimers from Diverse HIV-1 Isolates.

ABSTRACT:

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Soluble forms of trimeric HIV-1 envelope glycoprotein (Env) have long been sought as immunogens and as reagents for analysis of Env structure and function. Isolation of trimers that mimic native Env, derived from diverse viruses, however, represents a major challenge. Thus far, the most promising native-like (NL) structures have been obtained by engineering trimer-stabilizing mutations, termed SOSIP, into truncated Env sequences. However, the abundances of NL trimeric conformers vary among Envs, necessitating purification by monoclonal antibodies (MAbs) like PGT145, which target specific epitopes. To surmount this inherent limitation, we developed an approach that uses lectin affinity chromatography, ion-exchange chromatography, hydrophobic-interaction chromatography (HI

SUBMITTER: Verkerke HP 

PROVIDER: S-EPMC5044855 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature