Microbiome in the Apical Root Canal System of Teeth with Post-Treatment Apical Periodontitis.

ABSTRACT:

Introduction

Bacteria present in the apical root canal system are directly involved with the pathogenesis of post-treatment apical periodontitis. This study used a next-generation sequencing approach to identify the bacterial taxa occurring in cryopulverized apical root samples from root canal-treated teeth with post-treatment disease.

Methods

Apical root specimens obtained during periradicular surgery of ten adequately treated teeth with persistent apical periodontitis were cryogenically ground. DNA was extracted from the powder and the microbiome was characterized on the basis of the V4 hypervariable region of the 16S rRNA gene by using paired-end sequencing on Illumina MiSeq device.

Results

All samples were positive for the presence of bacterial DNA. Bacterial taxa

SUBMITTER: Siqueira JF 

PROVIDER: S-EPMC5045198 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature