Mitochondrial DNA content in breast cancer: Impact on in vitro and in vivo phenotype and patient prognosis.

ABSTRACT: Reduced mitochondrial DNA (mtDNA) content in breast cancer cell lines has been associated with transition towards a mesenchymal phenotype, but its clinical consequences concerning breast cancer dissemination remain unidentified. Here, we aimed to clarify the link between mtDNA content and a mesenchymal phenotype and its relation to prognosis of breast cancer patients. We analyzed mtDNA content in 42 breast cancer cell lines and 207 primary breast tumor specimens using a combination of quantitative PCR and array-based copy number analysis. By associating mtDNA content with expression levels of genes involved in epithelial-to-mesenchymal transition (EMT) and with the intrinsic breast cancer subtypes, we could not identify a relation between low mtDNA content and mesenchymal properties in the

SUBMITTER: Weerts MJ 

PROVIDER: S-EPMC5045386 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature