DSBCapture: in situ capture and sequencing of DNA breaks.

ABSTRACT: Double-strand DNA breaks (DSBs) continuously arise and cause mutations and chromosomal rearrangements. Here, we present DSBCapture, a sequencing-based method that captures DSBs in situ and directly maps these at single-nucleotide resolution, enabling the study of DSB origin. DSBCapture shows substantially increased sensitivity and data yield compared with other methods. Using DSBCapture, we uncovered a striking relationship between DSBs and elevated transcription within nucleosome-depleted chromatin.

SUBMITTER: Lensing SV 

PROVIDER: S-EPMC5045719 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature