Cytological Profile of Antibacterial FtsZ Inhibitors and Synthetic Peptide MciZ.

ABSTRACT: Cell division protein FtsZ is the organizer of the cytokinetic ring in almost all bacteria and a target for the discovery of new antibacterial agents that are needed to counter widespread antibiotic resistance. Bacterial cytological profiling, using quantitative microscopy, is a powerful approach for identifying the mechanism of action of antibacterial molecules affecting different cellular pathways. We have determined the cytological profile on Bacillus subtilis cells of a selection of small molecule inhibitors targeting FtsZ on different binding sites. FtsZ inhibitors lead to long undivided cells, impair the normal assembly of FtsZ into the midcell Z-rings, induce aberrant ring distributions, punctate FtsZ foci, membrane spots and also modify nucleoid length. Quantitative a

SUBMITTER: Araujo-Bazan L 

PROVIDER: S-EPMC5045927 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature