Soluble guanylate cyclase redox state under oxidative stress conditions in isolated monkey coronary arteries.

ABSTRACT: Coronary artery disease is associated with oxidative stress due to the excessive generation of free radicals in the vascular wall. This study investigated the impact of tert-butyl hydroperoxide (t-BuOOH), a peroxyl radical generator, on the redox state of soluble guanylate cyclase (sGC) in isolated monkey coronary arteries. Helically cut strips of endothelium-intact monkey coronary arteries treated with the nitric oxide synthase inhibitor N^G-nitro-L-arginine (10 μmol/L) were exposed for approximately 60 min to either no drug or t-BuOOH (100 μmol/L) in the presence and absence of α-tocopherol (300 μmol/L). Relaxation and cGMP levels in response to the sGC stimulator BAY 41-2272 and the sGC activator BAY 60-2770 were assessed by organ chamber technique

SUBMITTER: Tawa M 

PROVIDER: S-EPMC5045941 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature