A proteinaceous organic matrix regulates carbonate mineral production in the marine teleost intestine.

ABSTRACT: Marine teleost fish produce CaCO₃ in their intestine as part of their osmoregulatory strategy. This precipitation is critical for rehydration and survival of the largest vertebrate group on earth, yet the molecular mechanisms that regulate this reaction are unknown. Here, we isolate and characterize an organic matrix associated with the intestinal precipitates produced by Gulf toadfish (Opsanus beta). Toadfish precipitates were purified using two different methods, and the associated organic matrix was extracted. Greater than 150 proteins were identified in the isolated matrix by mass spectrometry and subsequent database searching using an O. beta transcriptomic sequence library produced here. Many of the identified proteins were enriched in the matrix compared to the intestinal

SUBMITTER: Schauer KL 

PROVIDER: S-EPMC5046086 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature