Efficient long-term cryopreservation of pluripotent stem cells at -80 °C.

ABSTRACT: Current long term cryopreservation of cell stocks routinely requires the use of liquid nitrogen (LN₂), because commonly used cryopreservation media containing cell membrane permeating cryoprotectants are thermally unstable when frozen at higher storage temperatures, e.g. -80 °C. This instability leads to ice recrystallization, causing progressive loss of cell viability over time under the storage conditions provided by most laboratory deep freezers. The dependency on LN₂ for cell storage significantly increases operational expense and raises issues related to impaired working efficiency and safety. Here we demonstrate that addition of Ficoll 70 to cryoprotectant solutions significantly improves system thermal stability at the working temperature (~-80 °C) of laborator

SUBMITTER: Yuan Y 

PROVIDER: S-EPMC5046093 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature