A sensitive electrochemical sensor for in vitro detection of parathyroid hormone based on a MoS₂-graphene composite.

ABSTRACT: This paper reports a biosensor based on a MoS₂-graphene (MG) composite that can measure the parathyroid hormone (PTH) concentration in serum samples from patients. The interaction between PTH and MG was analysed via an electrochemical sensing technique. The MG was functionalized using l-cysteine. Following this, PTH could be covalently immobilized on the MG sensing electrode. The properties of MG were evaluated using scanning electron microscopy, high-resolution transmission electron microscopy, X-ray diffraction, Raman spectroscopy, X-ray photoelectron spectroscopy, and Fourier transform infrared spectrometry. Following optimization of immobilized materials-such as MG, PTH, and alkaline phosphatase (ALP)-the performance of the MG sensor was investigated via cyclic voltammetry,

SUBMITTER: Kim HU 

PROVIDER: S-EPMC5046135 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature