High-throughput single-cell gene-expression profiling with multiplexed error-robust fluorescence in situ hybridization.

ABSTRACT: Image-based approaches to single-cell transcriptomics, in which RNA species are identified and counted in situ via imaging, have emerged as a powerful complement to single-cell methods based on RNA sequencing of dissociated cells. These image-based approaches naturally preserve the native spatial context of RNAs within a cell and the organization of cells within tissue, which are important for addressing many biological questions. However, the throughput of these image-based approaches is relatively low. Here we report advances that lead to a drastic increase in the measurement throughput of multiplexed error-robust fluorescence in situ hybridization (MERFISH), an image-based approach to single-cell transcriptomics. In MERFISH, RNAs are identified via a combinatorial labeling approach that

SUBMITTER: Moffitt JR 

PROVIDER: S-EPMC5047202 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature