Genetic and transcriptional analysis of human host response to healthy gut microbiota.

ABSTRACT: Many studies have demonstrated the importance of the gut microbiota in healthy and disease states. However, establishing the causality of host-microbiota interactions in humans is still challenging. Here, we describe a novel experimental system to define the transcriptional response induced by the microbiota in human cells and to shed light on the molecular mechanisms underlying host-gut microbiota interactions. In primary human colonic epithelial cells, we identified over 6,000 genes that change expression at various time points following co-culturing with the gut microbiota of a healthy individual. Among the differentially expressed genes we found a 1.8-fold enrichment of genes associated with diseases that have been previously linked to the microbiome, such as obesity and colorectal can

SUBMITTER: Richards AL 

PROVIDER: S-EPMC5047527 | biostudies-literature | 2016 Jul-Aug

REPOSITORIES: biostudies-literature