Comparison of Confocal and Super-Resolution Reflectance Imaging of Metal Oxide Nanoparticles.

ABSTRACT: The potential for human exposure to manufactured nanoparticles (NPs) has increased in recent years, in part through the incorporation of engineered particles into a wide range of commercial goods and medical applications. NP are ideal candidates for use as therapeutic and diagnostic tools within biomedicine, however concern exists regarding their efficacy and safety. Thus, developing techniques for the investigation of NP uptake into cells is critically important. Current intracellular NP investigations rely on the use of either Transmission Electron Microscopy (TEM), which provides ultrahigh resolution, but involves cumbersome sample preparation rendering the technique incompatible with live cell imaging, or fluorescent labelling, which suffers from photobleaching, poor bioconjugation and

SUBMITTER: Guggenheim EJ 

PROVIDER: S-EPMC5047631 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature