Distinct Cell Stress Responses Induced by ATP Restriction in Quiescent Human Fibroblasts.

ABSTRACT: Quiescence is the prevailing state of many cell types under homeostatic conditions. Yet, surprisingly little is known about how quiescent cells respond to energetic and metabolic challenges. To better understand compensatory responses of quiescent cells to metabolic stress, we established, in human primary dermal fibroblasts, an experimental 'energy restriction' model. Quiescence was achieved by short-term culture in serum-deprived media and ATP supply restricted using a combination of glucose transport inhibitors and mitochondrial uncouplers. In aggregate, these measures led to markedly reduced intracellular ATP levels while not compromising cell viability over the observation period of 48 h. Analysis of the transcription factor (TF) landscape induced by this treatment revealed alteration

SUBMITTER: Yalamanchili N 

PROVIDER: S-EPMC5047886 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature