Validation of tumor protein marker quantification by two independent automated immunofluorescence image analysis platforms.

ABSTRACT: Protein marker levels in formalin-fixed, paraffin-embedded tissue sections traditionally have been assayed by chromogenic immunohistochemistry and evaluated visually by pathologists. Pathologist scoring of chromogen staining intensity is subjective and generates low-resolution ordinal or nominal data rather than continuous data. Emerging digital pathology platforms now allow quantification of chromogen or fluorescence signals by computer-assisted image analysis, providing continuous immunohistochemistry values. Fluorescence immunohistochemistry offers greater dynamic signal range than chromogen immunohistochemistry, and combined with image analysis holds the promise of enhanced sensitivity and analytic resolution, and consequently more robust quantification. However, commercial fluorescenc

SUBMITTER: Peck AR 

PROVIDER: S-EPMC5047958 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature