Optimized Collection Protocol for Plasma MicroRNA Measurement in Patients with Cardiovascular Disease.

ABSTRACT: Background. Various microRNAs (miRNAs) are used as markers of acute coronary syndrome, in which heparinization is considered mandatory therapy. Nevertheless, a standard method of handling plasma samples has not been proposed, and the effects of heparin treatment on miRNA detection are rarely discussed. Materials and Method. This study used quantitative polymerase chain reaction (qPCR) analysis to investigate how storage temperature, standby time, hemolysis, and heparin treatment affect miRNA measurement in plasma samples from 25 patients undergoing cardiac catheterization. Results. For most miRNAs, the qPCR results remained consistent during the first 2 hours. The miRNA signals did not significantly differ between samples stored at 4°C before processing and samples sto

SUBMITTER: Wu CS 

PROVIDER: S-EPMC5048034 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature