Photodynamic N-TiO₂ Nanoparticle Treatment Induces Controlled ROS-mediated Autophagy and Terminal Differentiation of Leukemia Cells.

ABSTRACT: In this study, we used nitrogen-doped titanium dioxide (N-TiO₂) NPs in conjugation with visible light, and show that both reactive oxygen species (ROS) and autophagy are induced by this novel NP-based photodynamic therapy (PDT) system. While well-dispersed N-TiO₂ NPs (≤100 μg/ml) were inert, their photo-activation with visible light led to ROS-mediated autophagy in leukemia K562 cells and normal peripheral lymphocytes, and this increased in parallel with increasing NP concentrations and light doses. At a constant light energy (12 J/cm²), increasing N-TiO₂ NP concentrations increased ROS levels to trigger autophagy-dependent megakaryocytic terminal differentiation in K562 cells. By contrast, an ROS challenge induced by high N-TiO₂ NP co

SUBMITTER: Moosavi MA 

PROVIDER: S-EPMC5048164 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature