Capture-C reveals preformed chromatin interactions between HIF-binding sites and distant promoters.

ABSTRACT: Hypoxia-inducible factor (HIF) directs an extensive transcriptional cascade that transduces numerous adaptive responses to hypoxia. Pan-genomic analyses, using chromatin immunoprecipitation and transcript profiling, have revealed large numbers of HIF-binding sites that are generally associated with hypoxia-inducible transcripts, even over long chromosomal distances. However, these studies do not define the specific targets of HIF-binding sites and do not reveal how induction of HIF affects chromatin conformation over distantly connected functional elements. To address these questions, we deployed a recently developed chromosome conformation assay that enables simultaneous high-resolution analyses from multiple viewpoints. These assays defined specific long-range interactions between interg

SUBMITTER: Platt JL 

PROVIDER: S-EPMC5048371 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature