Measuring cell surface area and deformability of individual human red blood cells over blood storage using quantitative phase imaging.

ABSTRACT: The functionality and viability of stored human red blood cells (RBCs) is an important clinical issue in transfusions. To systematically investigate changes in stored whole blood, the hematological properties of individual RBCs were quantified in blood samples stored for various periods with and without a preservation solution called citrate phosphate dextrose adenine-1 (CPDA-1). With 3-D quantitative phase imaging techniques, the optical measurements for 3-D refractive index (RI) distributions and membrane fluctuations were done at the individual cell level. From the optical measurements, the morphological (volume, surface area and sphericity), biochemical (hemoglobin content and concentration), and mechanical parameters (dynamic membrane fluctuation) were simultaneously quantified to inv

SUBMITTER: Park H 

PROVIDER: S-EPMC5048416 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature