RNA Structural Modules Control the Rate and Pathway of RNA Folding and Assembly.

ABSTRACT: Structured RNAs fold through multiple pathways, but we have little understanding of the molecular features that dictate folding pathways and determine rates along a given pathway. Here, we asked whether folding of a complex RNA can be understood from its structural modules. In a two-piece version of the Tetrahymena group I ribozyme, the separated P5abc subdomain folds to local native secondary and tertiary structure in a linked transition and assembles with the ribozyme core via three tertiary contacts: a kissing loop (P14), a metal core-receptor interaction, and a tetraloop-receptor interaction, the first two of which are expected to depend on native P5abc structure from the local transition. Native gel, NMR, and chemical footprinting experiments showed that mutations that destabilize the

SUBMITTER: Gracia B 

PROVIDER: S-EPMC5048535 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature