The Replicative Consequences of Papillomavirus E2 Protein Binding to the Origin Replication Factor ORC2.

ABSTRACT: The origin recognition complex (ORC) coordinates a series of events that lead to initiation of DNA strand duplication. As a nuclear double stranded DNA plasmid, the papillomavirus (PV) genome resembles a mini-chromosome in infected cells. To initiate its replication, the viral E2 protein binds to and recruits the E1 DNA helicase at the viral origin. PV genome replication program exhibits three stages: initial amplification from a single genome upon infection to a few copies per cell, a cell cycle linked maintenance phase, and a differentiation dependent late stage where the genome is amplified to thousands of copies. Involvement of ORC or other pre-replication complex (pre-RC) factors has not been described. We report that human PV (HPV) and bovine PV (BPV-1) E2 proteins bind to ORC2, howe

SUBMITTER: DeSmet M 

PROVIDER: S-EPMC5049798 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature