Sickle Cell Trait Increases Red Blood Cell Storage Hemolysis and Post-Transfusion Clearance in Mice.

ABSTRACT:

Background

Transfusion of blood at the limits of approved storage time is associated with lower red blood cell (RBC) post-transfusion recovery and hemolysis, which increases plasma cell-free hemoglobin and iron, proposed to induce endothelial dysfunction and impair host defense. There is noted variability among donors in the intrinsic rate of storage changes and RBC post-transfusion recovery, yet genetic determinants that modulate this process are unclear.

Methods

We explore RBC storage stability and post-transfusion recovery in murine models of allogeneic and xenogeneic transfusion using blood from humanized transgenic sickle cell hemizygous mice (Hba^tm1PazHbb^tm1TowTg(HBA-HBBs)41Paz/J) and human donors with a common genetic mutation sickle cell trait (

SUBMITTER: Osei-Hwedieh DO 

PROVIDER: S-EPMC5049931 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature