ImmunoPET for assessing the differential uptake of a CD146-specific monoclonal antibody in lung cancer.

ABSTRACT:

Purpose

Overexpression of CD146 in solid tumors has been linked to disease progression, invasion, and metastasis. We describe the generation of a ⁶⁴Cu-labeled CD146-specific antibody and its use for quantitative immunoPET imaging of CD146 expression in six lung cancer models.

Methods

The anti-CD146 antibody (YY146) was conjugated to 1,4,7-triazacyclononane-triacetic acid (NOTA) and radiolabeled with ⁶⁴Cu. CD146 expression was evaluated in six human lung cancer cell lines (A549, NCI-H358, NCI-H522, HCC4006, H23, and NCI-H460) by flow cytometry and quantitative western blot studies. The biodistribution and tumor uptake of ⁶⁴Cu-NOTA-YY146 was assessed by sequential PET imaging in athymic nude mice bearing subcutaneous lung cancer xenografts. The

SUBMITTER: Sun H 

PROVIDER: S-EPMC5050101 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature