Diversification of Gene Expression during Formation of Static Submerged Biofilms by Escherichia coli.

ABSTRACT: Many bacteria primarily exist in nature as structured multicellular communities, so called biofilms. Biofilm formation is a highly regulated process that includes the transition from the motile planktonic to sessile biofilm lifestyle. Cellular differentiation within a biofilm is a commonly accepted concept but it remains largely unclear when, where and how exactly such differentiation arises. Here we used fluorescent transcriptional reporters to quantitatively analyze spatio-temporal expression patterns of several groups of genes during the formation of submerged Escherichia coli biofilms in an open static system. We first confirm that formation of such submerged biofilms as well as pellicles at the liquid-air interface requires the major matrix component, curli, and flagella-mediat

SUBMITTER: Besharova O 

PROVIDER: S-EPMC5050211 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature