Fast and Efficient Transfection of Mouse Embryonic Stem Cells Using Non-Viral Reagents.

ABSTRACT: Reliable and efficient DNA and RNA transfection methods are required when studying the role of individual genes in mouse pluripotent stem cells. However, these cells usually grow in tight clusters and are therefore more difficult to transfect than many other cell lines. We have found that transfection is especially challenging when mouse embryonic stem (mES) cells are cultured in the newly described 2i medium, which is based on two chemical inhibitors of differentiation pathways. In the present study we have performed a side-by-side comparison of commercially available, non-viral transfection reagents with regard to their ability to deliver plasmid DNA and siRNA into adherent and/or trypsinized mES cells cultured in 2i medium, assessing transfection rates, plasmid gene expression, siRNA me

SUBMITTER: Tamm C 

PROVIDER: S-EPMC5050252 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature