Rapid detection of abrin in foods with an up-converting phosphor technology-based lateral flow assay.

ABSTRACT: Abrin is a natural plant toxin found in the seeds of Abrus precatorius. It may be used for food poisoning or bioterrorism, seriously endangering public health. In this study, a reliable method for the rapid detection of abrin in foods was developed, based on an up-converting phosphor technology-based lateral flow assay (abrin-UPT-LFA). Nine high-affinity monoclonal antibodies (mAbs) against abrin were prepared, and the optimum mAbs (mAb-6F4 and mAb-10E11) were selected for use in the assay in double-antibody-sandwich mode. The assay was confirmed to be specific for abrin, with a detection sensitivity of 0.1 ng mL^-1 for standard abrin solutions. Good linearity was observed for abrin quantitation from 0.1 to 1000 ng mL^-1 (r = 0.9983). During the analysis of various abri

SUBMITTER: Liu X 

PROVIDER: S-EPMC5050493 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature