Mining gut microbiome oligopeptides by functional metaproteome display.

ABSTRACT: Pathogen infections, autoimmune diseases, and chronic inflammatory disorders are associated with systemic antibody responses from the host immune system. Disease-specific antibodies can be important serum biomarkers, but the identification of antigens associated with specific immune reactions is challenging, in particular if complex communities of microorganisms are involved in the disease progression. Despite promising new diagnostic opportunities, the discovery of these serological markers becomes more difficult with increasing complexity of microbial communities. In the present work, we used a metagenomic M13 phage display approach to select immunogenic oligopeptides from the gut microbiome of transgenic mice suffering from chronic ileitis. We constructed three individual metaproteome p

SUBMITTER: Zantow J 

PROVIDER: S-EPMC5050496 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature