Second-generation probes for biosynthetic intermediate capture: towards a comprehensive profiling of polyketide assembly.

ABSTRACT: Malonyl carba(dethia) N-decanoyl cysteamine methyl esters and novel acetoxymethyl esters were utilised as second-generation probes for polyketide intermediate capture. The use of these tools in vivo led to the characterisation of an almost complete set of biosynthetic intermediates from a modular assembly line, providing a first kinetic overview of intermediate processing leading to complex natural product formation.

SUBMITTER: Wilkening I 

PROVIDER: S-EPMC5050551 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature