Enzymatic fine-tuning for 2-(6-hydroxynaphthyl) β-D-xylopyranoside synthesis catalyzed by the recombinant β-xylosidase BxTW1 from Talaromyces amestolkiae.

ABSTRACT:

Background

Glycosides are compounds displaying crucial biological roles and plenty of applications. Traditionally, these molecules have been chemically obtained, but its efficient production is limited by the lack of regio- and stereo-selectivity of the chemical synthesis. As an interesting alternative, glycosidases are able to catalyze the formation of glycosides in a process considered green and highly selective. In this study, we report the expression and characterization of a fungal β-xylosidase in Pichia pastoris. The transglycosylation potential of the enzyme was evaluated and its applicability in the synthesis of a selective anti-proliferative compound demonstrated.

Results

The β-xylosidase BxTW1 from the ascomycete fungus Talaromyces amestolkiae was cloned and expres

SUBMITTER: Nieto-Dominguez M 

PROVIDER: S-EPMC5050587 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature