Novel microRNA discovery using small RNA sequencing in post-mortem human brain.

ABSTRACT:

Background

MicroRNAs (miRNAs) are short, non-coding RNAs that regulate gene expression mainly through translational repression of target mRNA molecules. More than 2700 human miRNAs have been identified and some are known to be associated with disease phenotypes and to display tissue-specific patterns of expression.

Methods

We used high-throughput small RNA sequencing to discover novel miRNAs in 93 human post-mortem prefrontal cortex samples from individuals with Huntington's disease (n = 28) or Parkinson's disease (n = 29) and controls without neurological impairment (n = 36). A custom miRNA identification analysis pipeline was built, which utilizes miRDeep* miRNA identification and result filtering based on false positive rate estimates.

Results

Ninety-nine novel miR

SUBMITTER: Wake C 

PROVIDER: S-EPMC5050850 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature