A Novel Self-Assembling DNA Nano Chip for Rapid Detection of Human Papillomavirus Genes.

ABSTRACT: Rapid detection of tumor-associated DNA such as Human Papillomavirus (HPV) has important clinical value for the early screening of tumors. By attaching oligonucleotides or cDNA onto the chip surface, DNA chip technology provides a rapid method to analyze gene expression. However, challenges remain regarding increasing probe density and improving detection time. To address these challenges, we proposed a DNA chip that was self-assembled from single stranded DNA in combination with high probe density and a rapid detection method. Over 200 probes could be attached to the surface of this 100-nm diameter DNA chip. For detection, the chips were adsorbed onto a mica surface and then incubated for ten minutes with HPV-DNA; the results were directly observable using atomic force microscopy (AFM). T

SUBMITTER: Li X 

PROVIDER: S-EPMC5051682 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature