CENPT bridges adjacent CENPA nucleosomes on young human α-satellite dimers.

ABSTRACT: Nucleosomes containing the CenH3 (CENPA or CENP-A) histone variant replace H3 nucleosomes at centromeres to provide a foundation for kinetochore assembly. CENPA nucleosomes are part of the constitutive centromere associated network (CCAN) that forms the inner kinetochore on which outer kinetochore proteins assemble. Two components of the CCAN, CENPC and the histone-fold protein CENPT, provide independent connections from the ∼171-bp centromeric α-satellite repeat units to the outer kinetochore. However, the spatial relationship between CENPA nucleosomes and these two branches remains unclear. To address this issue, we use a base-pair resolution genomic readout of protein-protein interactions, comparative chromatin immunoprecipitation (ChIP) with sequencing, together with sequential ChIP, t

SUBMITTER: Thakur J 

PROVIDER: S-EPMC5052034 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature