Gene discovery by chemical mutagenesis and whole-genome sequencing in Dictyostelium.

ABSTRACT: Whole-genome sequencing is a useful approach for identification of chemical-induced lesions, but previous applications involved tedious genetic mapping to pinpoint the causative mutations. We propose that saturation mutagenesis under low mutagenic loads, followed by whole-genome sequencing, should allow direct implication of genes by identifying multiple independent alleles of each relevant gene. We tested the hypothesis by performing three genetic screens with chemical mutagenesis in the social soil amoeba Dictyostelium discoideum Through genome sequencing, we successfully identified mutant genes with multiple alleles in near-saturation screens, including resistance to intense illumination and strong suppressors of defects in an allorecognition pathway. We tested the causality of the muta

SUBMITTER: Li CL 

PROVIDER: S-EPMC5052037 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature