Single-cell analyses of X Chromosome inactivation dynamics and pluripotency during differentiation.

ABSTRACT: Pluripotency, differentiation, and X Chromosome inactivation (XCI) are key aspects of embryonic development. However, the underlying relationship and mechanisms among these processes remain unclear. Here, we systematically dissected these features along developmental progression using mouse embryonic stem cells (mESCs) and single-cell RNA sequencing with allelic resolution. We found that mESCs grown in a ground state 2i condition displayed transcriptomic profiles diffused from preimplantation mouse embryonic cells, whereas EpiStem cells closely resembled the post-implantation epiblast. Sex-related gene expression varied greatly across distinct developmental states. We also identified novel markers that were highly enriched in each developmental state. Moreover, we revealed that several nov

SUBMITTER: Chen G 

PROVIDER: S-EPMC5052059 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature