MR molecular imaging of tumours using ferritin heavy chain reporter gene expression mediated by the hTERT promoter.

ABSTRACT:

Objectives

Using the human telomerase reverse transcriptase (hTERT) promoter and the modified ferritin heavy chain (Fth) reporter gene, reporter gene expression for MRI was examined in telomerase positive and negative tumour cells and xenografts.

Methods

Activity of the reporter gene expression vector Lenti-hTERT-Fth1-3FLAG-Puro was compared to constitutive CMV-driven expression and to the untransfected parental control in five tumour cell lines: A549, SKOV3, 293T, U2OS and HPDLF. In vitro, transfected cells were evaluated for FLAG-tagged protein expression, iron accumulation and transverse relaxation. In vivo, tumours transduced by lentiviral vector injection were imaged using T2*WI. Changes in tumour signal intensity were validated by histology.

Results

Only telomer

SUBMITTER: Yang Y 

PROVIDER: S-EPMC5052315 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature