Single-Molecule Analysis beyond Dwell Times: Demonstration and Assessment in and out of Equilibrium.

ABSTRACT: We present a simple and robust technique for extracting kinetic rate models and thermodynamic quantities from single-molecule time traces. Single-molecule analysis of complex kinetic sequences (SMACKS) is a maximum-likelihood approach that resolves all statistically relevant rates and also their uncertainties. This is achieved by optimizing one global kinetic model based on the complete data set while allowing for experimental variations between individual trajectories. In contrast to dwell-time analysis, which is the current standard method, SMACKS includes every experimental data point, not only dwell times. As a result, it works as well for long trajectories as for an equivalent set of short ones. In addition, the previous systematic overestimation of fast over slow rates is solved. We

SUBMITTER: Schmid S 

PROVIDER: S-EPMC5052450 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature