Diaphanous gene mutation affects spiral cleavage and chirality in snails.

ABSTRACT: L-R (left and right) symmetry breaking during embryogenesis and the establishment of asymmetric body plan are key issues in developmental biology, but the onset including the handedness-determining gene locus still remains unknown. Using pure dextral (DD) and sinistral (dd) strains of the pond snail Lymnaea stagnalis as well as its F₂ through to F₁₀ backcrossed lines, the single handedness-determining-gene locus was mapped by genetic linkage analysis, BAC cloning and chromosome walking. We have identified the actin-related diaphanous gene Lsdia1 as the strongest candidate. Although the cDNA and derived amino acid sequences of the tandemly duplicated Lsdia1 and Lsdia2 genes are very similar, we could discriminate the two genes/proteins in our molecular biology experime

SUBMITTER: Kuroda R 

PROVIDER: S-EPMC5052593 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature