Single-molecule imaging of electroporated dye-labelled CheY in live Escherichia coli.

ABSTRACT: For the past two decades, the use of genetically fused fluorescent proteins (FPs) has greatly contributed to the study of chemotactic signalling in Escherichia coli including the activation of the response regulator protein CheY and its interaction with the flagellar motor. However, this approach suffers from a number of limitations, both biological and biophysical: for example, not all fusions are fully functional when fused to a bulky FP, which can have a similar molecular weight to its fused counterpart; they may interfere with the native interactions of the protein and the chromophores of FPs have low brightness and photostability and fast photobleaching rates. A recently developed technique for the electroporation of fluorescently labelled proteins in live bacteria has enabled us to b

SUBMITTER: Di Paolo D 

PROVIDER: S-EPMC5052738 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature