Assessment of CYP1A2 enzyme activity in relation to type-2 diabetes and habitual caffeine intake.

ABSTRACT:

Background

Coffee consumption is a known inducer of cytochrome P450 1A2 (CYP1A2) enzyme activity. We recently observed that a group of type-2 diabetes patients consumed more caffeine (coffee) on a daily basis than non-type-2 diabetes controls. Here, we investigated whether type-2 diabetes cases may metabolize caffeine faster than non-type-2 diabetes controls.

Methods

To estimate CYP1A2 enzyme activity, an established marker of caffeine metabolism, we quantified the paraxanthine/caffeine concentration ratio in saliva in 57 type-2 diabetes and 146 non-type-2 diabetes participants in a case-control field study. All participants completed validated questionnaires regarding demographic status, health and habitual caffeine intake, and were genotyped for the functional -163C > A po

SUBMITTER: Urry E 

PROVIDER: S-EPMC5052791 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature