Proteomic and metabolomic analyses reveal metabolic responses to 3-hydroxypropionic acid synthesized internally in cyanobacterium Synechocystis sp. PCC 6803.

ABSTRACT:

Background

3-hydroxypropionic acid (3-HP) is an important platform chemical with a wide range of applications. In our previous study, the biosynthetic pathway of 3-HP was constructed and optimized in cyanobacterium Synechocystis sp. PCC 6803, which led to 3-HP production directly from CO₂ at a level of 837.18 mg L^-1 (348.8 mg/g dry cell weight). As the production and accumulation of 3-HP in cells affect cellular metabolism, a better understanding of cellular responses to 3-HP synthesized internally in Synechocystis will be important for further increasing 3-HP productivity in cyanobacterial chassis.

Results

Using a engineered 3-HP-producing SM strain, in this study, the cellular responses to 3-HP internally produced were first determined u

SUBMITTER: Wang Y 

PROVIDER: S-EPMC5053081 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature