Intraocular Pressure Induced Retinal Changes Identified Using Synchrotron Infrared Microscopy.

ABSTRACT: Infrared (IR) spectroscopy has been used to quantify chemical and structural characteristics of a wide range of materials including biological tissues. In this study, we examined spatial changes in the chemical characteristics of rat retina in response to intraocular pressure (IOP) elevation using synchrotron infrared microscopy (SIRM), a non-destructive imaging approach. IOP elevation was induced by placing a suture around the eye of anaesthetised rats. Retinal sections were collected onto transparent CaF2 slides 10 days following IOP elevation. Using combined SIRM spectra and chemical mapping approaches it was possible to quantify IOP induced changes in protein conformation and chemical distribution in various layers of the rat retina. We showed that 10 days following IOP elevation there

SUBMITTER: Shen HH 

PROVIDER: S-EPMC5053542 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature