Read-through transcripts in normal human lung parenchyma are down-regulated in lung adenocarcinoma.

ABSTRACT: Read-through transcripts result from the continuous transcription of adjacent, similarly oriented genes, with the splicing out of the intergenic region. They have been found in several neoplastic and normal tissues, but their pathophysiological significance is unclear. We used high-throughput sequencing of cDNA fragments (RNA-Seq) to identify read-through transcripts in the non-involved lung tissue of 64 surgically treated lung adenocarcinoma patients. A total of 52 distinct read-through species was identified, with 24 patients having at least one read-through event, up to a maximum of 17 such transcripts in one patient. Sanger sequencing validated 28 of these transcripts and identified an additional 15, for a total of 43 distinct read-through events involving 35 gene pairs. Expression lev

SUBMITTER: Pintarelli G 

PROVIDER: S-EPMC5053695 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature