High-sensitivity transcriptome data structure and implications for analysis and biologic interpretation.

ABSTRACT: Novel microarray technologies such as the AB1700 platform from Applied Biosystems promise significant increases in the signal dynamic range and a higher sensitivity for weakly expressed transcripts. We have compared a representative set of AB1700 data with a similarly representative Affymetrix HG-U133A dataset. The AB1700 design extends the signal dynamic detection range at the lower bound by one order of magnitude. The lognormal signal distribution profiles of these high-sensitivity data need to be represented by two independent distributions. The additional second distribution covers those transcripts that would have gone undetected using the Affymetrix technology. The signal-dependent variance distribution in the AB1700 data is a non-trivial function of signal intensity, describable usi

SUBMITTER: Noth S 

PROVIDER: S-EPMC5054080 | biostudies-literature | 2006 Nov

REPOSITORIES: biostudies-literature