Phosphoproteomics to Characterize Host Response During Influenza A Virus Infection of Human Macrophages.

ABSTRACT: Influenza A viruses cause infections in the human respiratory tract and give rise to annual seasonal outbreaks, as well as more rarely dreaded pandemics. Influenza A viruses become quickly resistant to the virus-directed antiviral treatments, which are the current main treatment options. A promising alternative approach is to target host cell factors that are exploited by influenza viruses. To this end, we characterized the phosphoproteome of influenza A virus infected primary human macrophages to elucidate the intracellular signaling pathways and critical host factors activated upon influenza infection. We identified 1675 phosphoproteins, 4004 phosphopeptides and 4146 nonredundant phosphosites. The phosphorylation of 1113 proteins (66%) was regulated upon infection, highlighting the impor

SUBMITTER: Soderholm S 

PROVIDER: S-EPMC5054344 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature