Quantitative Profiling of Single Formalin Fixed Tumour Sections: proteomics for translational research.

ABSTRACT: Although re-sequencing of gene panels and mRNA expression profiling are now firmly established in clinical laboratories, in-depth proteome analysis has remained a niche technology, better suited for studying model systems rather than challenging materials such as clinical trial samples. To address this limitation, we have developed a novel and optimized platform called SP3-Clinical Tissue Proteomics (SP3-CTP) for in-depth proteome profiling of practical quantities of tumour tissues, including formalin fixed and paraffin embedded (FFPE). Using single 10 μm scrolls of clinical tumour blocks, we performed in-depth quantitative analyses of individual sections from ovarian tumours covering the high-grade serous, clear cell, and endometrioid histotypes. This examination enabled the generation of

SUBMITTER: Hughes CS 

PROVIDER: S-EPMC5054533 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature