Hematopoietic Stem Cells Are the Major Source of Multilineage Hematopoiesis in Adult Animals.

ABSTRACT: Hematopoietic stem cells (HSCs) sustain long-term reconstitution of hematopoiesis in transplantation recipients, yet their role in the endogenous steady-state hematopoiesis remains unclear. In particular, recent studies suggested that HSCs provide a relatively minor contribution to immune cell development in adults. We directed transgene expression in a fraction of HSCs that maintained reconstituting activity during serial transplantations. Inducible genetic labeling showed that transgene-expressing HSCs gave rise to other phenotypic HSCs, confirming their top position in the differentiation hierarchy. The labeled HSCs rapidly contributed to committed progenitors of all lineages and to mature myeloid cells and lymphocytes, but not to B-1a cells or tissue macrophages. Importantly, labeled H

SUBMITTER: Sawai CM 

PROVIDER: S-EPMC5054720 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature