An In Vitro Perfusion System to Enhance Outflow Studies in Mouse Eyes.

ABSTRACT:

Purpose

The molecular mechanisms controlling aqueous humor (AQH) outflow and IOP need much further definition. The mouse is a powerful system for characterizing the mechanistic basis of AQH outflow. To enhance outflow studies in mice, we developed a perfusion system that is based on human anterior chamber perfusion culture systems. Our mouse system permits previously impractical experiments.

Methods

We engineered a computer-controlled, pump-based perfusion system with a platform for mounting whole dissected mouse eyes (minus lens and iris, ∼45% of drainage tissue is perfused). We tested the system's ability to monitor outflow and tested the effects of the outflow-elevating drug, Y27632, a rho-associated protein kinase (ROCK) inhibitor. Finally, we tested the system's ability

SUBMITTER: Kizhatil K 

PROVIDER: S-EPMC5054733 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature